Donkey Anti-Goat IgG H&L (HRP) ab6885 from Abcam

Product Specs
ItemDonkey Anti-Goat IgG H&L (HRP)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab6885
Quantity1 mg
HostDonkey
ApplicationsDot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Immunomicroscopy, Western blot
Conjugate/TagHRP
Antibody TypeSecondary
FormatThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Concentration2 mg/ml
TargetIgG
ClonalityPolyclonal

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Cistanche deserticola Polysaccharide Reduces Inflammation and Aging Phenotypes in the Dermal Fibroblasts through the Activation of the NRF2/HO-1 PathwayInternational Journal of Molecular SciencesOctober 28, 2023Kento Takaya, Toru Asou, Kazuo Kishithe membranes were incubated with the following secondary antibodies; donkey anti-goat IgG H&L (HRP) (ab6885; 1:5000 dilution, Abcam), goat anti-rabbit IgG H&L (HRP) (ab205718; Abcam), goat anti-mouse IgG H&L (HRP

(2) CXCL13 promotes thermogenesis in mice via recruitment of M2 macrophage and inhibition of inflammation in brown adipose tissueFrontiers in ImmunologyOctober 23, 2023Lijun Xie, Huiying Wang, Dan Wu, Feng Zhang, Wei Chen, Yuqing Ye, Fang Hupre-adipocyte cell medium (CM) was added to lower chambers with CXCL13, anti-CXCL13 antibody or anti-goat IgG (ab6885, Abcam, USA), and the upper chambers contained serum-free DMEM with approximately 2*10^5 BMDM or RAW264.7

(3) Cholinergic signaling influences the expression of immune checkpoint inhibitors, PD-L1 and PD-L2, and tumor hallmarks in human colorectal cancer tissues and cell linesBMC cancerOctober 12, 2023Kuol N, Godlewski J, Kmiec Z, Vogrin S, Fraser S, Apostolopoulos V, Nurgali K.dilution of 1:10,000 [anti-mouse (Abcam, ab6789), anti-rabbit (Abcam, ab6721) and anti-goat (Abcam, ab6885)] for 2 h at room temperature. The membranes were washed in PBS plus 0.1% Tween 20, and protein detection

(4) Single-cell RNA sequencing of neurofibromas reveals a tumor microenvironment favorable for neural regeneration and immune suppression in a neurofibromatosis type 1 porcine modelFrontiers in OncologySeptember 25, 2023Dalton T. McLean, Jennifer J. Meudt, Loren D. Lopez Rivera, Dominic T. Schomberg, Derek M. Pavelec, Tyler T. Duellman, Darya G. Buehler, Patrick B. Schwartz, Melissa Graham, Laura M. Lee, et al.while the secondary antibodies, including anti-rabbit, anti-goat, and anti-mouse (1:250, Abcam; ab6728, ab6885, ab6721, respectively), were incubated on slides for 30 minutes at room temperature. The targets of

(5) Asiaticoside ameliorates renal ischemia/reperfusion injury by promoting CD4+CD25+FOXP3+ treg cell differentiationHeliyonJuly 1, 2023Shengjie Tang, Xiangcheng Xie, Ming Wang, Wei Wei(ab6721; 1:20,000, Abcam), goat-anti mouse IgG (ab6789; 1:10,000, Abcam), or donkey-anti goat IgG (ab6885; 1:10,000, Abcam) antibody for 2 h. At last, after the membrane was added with ECL Luminescent Liquid

(6) Elucidation of HHEX in pancreatic endoderm differentiation using a human iPSC differentiation modelScientific reportsMay 29, 2023Ito R, Kimura A, Hirose Y, Hatano Y, Mima A, Mae SI, Keidai Y, Nakamura T, Fujikura J, Nishi Y, et al.conjugated donkey anti-rabbit (Cytiva, NA934VS, 1:3000) and HRP conjugated donkey anti-goat (Abcam, ab6885, 1:5000). After washing with TBS-T, the membrane was incubated with ECL Western Blotting Detection Reagent

(7) Compound 13 Promotes Epidermal Healing in Mouse Fetuses via Activation of AMPKBiomedicinesMarch 27, 2023Kento Takaya, Keisuke Okabe, Shigeki Sakai, Noriko Aramaki-Hattori, Toru Asou, Kazuo Kishiincubated with the following secondary antibodies at 37 °C for 1 h: donkey anti-goat IgG H&L (HRP) (ab6885; Abcam) and goat anti-rabbit IgG H&L (HRP) (ab205718; Abcam). After washing, immunoreactive protein

(8) Virus-free transfection, transient expression, and purification of human cardiac myosin in mammalian muscle cells for biochemical and biophysical assaysScientific ReportsMarch 12, 2023Lok Priya Velayuthan, Luisa Moretto, Sven Tågerud, Marko Ušaj, Alf Månssonblocking buffer. The blot was washed in TBS-T buffer and then incubated with Donkey anti-goat antibodies (ab6885, Abcam) diluted in TBS-T at 1:20,000 for an hour. The membrane was finally washed with TBS-T and the

(9) Chronic spinal cord compression associated with intervertebral disc degeneration in SPARC-null miceNeural regeneration researchMarch 1, 2023Li ZY, Zhou AF, Li G, Zhou LY, Pu PM, Zhu K, Zheng Z, Wang YJ, Liang QQ, Yao M, et al.Beyotime Biotechnology, Cat# A0216, RRID: AB_2860575) and donkey anti-goat IgG (1:2000, Abcam, Cat# ab6885, RRID: AB_955423) for 1 hour at 20–25°C. The membrane was rinsed three times with Tris-buffered saline

(10) Cathepsin F is a potential marker for senescent human skin fibroblasts and keratinocytes associated with skin agingGeroScienceFebruary 1, 2023Kento Takaya, Toru Asou, Kazuo Kishithe samples were incubated with the following secondary antibodies: donkey anti-goat IgG H&L (HRP) (ab6885; Abcam), goat anti-rabbit IgG H&L (HRP) (ab205718; Abcam), and goat anti-mouse IgG H&L (HRP) (ab205719;

(11) Blocking Muscarinic Receptor 3 Attenuates Tumor Growth and Decreases Immunosuppressive and Cholinergic Markers in an Orthotopic Mouse Model of Colorectal CancerInternational journal of molecular sciencesDecember 29, 2022Kuol N, Davidson M, Karakkat J, Filippone RT, Veale M, Luwor R, Fraser S, Apostolopoulos V, Nurgali K.dilution of 1:10,000 [anti-mouse (Abcam, ab6789), anti-rabbit (Abcam, ab6721), and anti-goat (Abcam, ab6885)] for 2 h at room temperature followed by three times PBS-0.1% Tween 20 washes. Glyceraldehydes-3-phosphate

(12) Glutaminase inhibitors rejuvenate human skin via clearance of senescent cells: a study using a mouse/human chimeric modelAging (Albany NY)November 30, 2022Kento Takaya, Tatsuyuki Ishii, Toru Asou, Kazuo Kishiwashing with TBST, the cells were incubated with secondary antibodies [donkey anti-goat IgG H&L (HRP) (ab6885; Abcam), goat anti-rabbit IgG H&L (HRP) (ab205718; Abcam), and goat anti-mouse IgG H&L (HRP) (ab205719;

(13) Aging Fibroblasts Adversely Affect Extracellular Matrix Formation via the Senescent Humoral Factor Ependymin-Related Protein 1CellsNovember 24, 2022Takaya K, Asou T, Kishi K.solution at 4 °C overnight. The next day, the samples were incubated with donkey anti-goat IgG H&L (HRP) (ab6885; Abcam) and goat anti-rabbit IgG H&L (HRP) (ab205718; Abcam) at a 1:1000 dilution at 37 °C for 2 h.

(14) Olfactory marker protein contains a leucine-rich domain in the Ω-loop important for nuclear exportMolecular brainNovember 4, 2022Nakashima N, Nakashima A, Nakashima K, Takano M.peroxidase (HRP; 7074P2, Cell Signaling Technology), an anti-goat HRP-conjugated secondary antibody (ab6885, Abcam) or an anti-mouse HRP-conjugated secondary antibody (ab6823, Abcam, Cambridge, UK) all at a 1:2000

(15) Heterozygous expression of Cre recombinase in podocytes has no impact on the anti‐glomerular basement membrane glomerulonephritis model in C57BL / 6J micePhysiological ReportsSeptember 9, 2022Cyril Mousseaux, Tiffany Migeon, Perrine Frère, Marie Christine Verpont, Elisabeth Lutete, Claire Navarro, Liliane Louedec, Juliette Hadchouelperoxidase‐conjugated secondary antibodies [donkey anti‐rabbit 1/5000 (NA9340, Amersham); donkey anti‐Goat 1/5000 (ab6885, Abcam)]. The resulting bands were visualized by enhanced chemiluminescence (Clarity Western ECL substrate;

(16) Downregulation of senescence-associated secretory phenotype by knockdown of secreted frizzled-related protein 4 contributes to the prevention of skin agingAgingSeptember 7, 2022Takaya K, Asou T, Kishi K.the sections were incubated with the following secondary antibodies: donkey anti-goat IgG H&L (HRP) (ab6885; Abcam), goat anti-rabbit IgG H&L (HRP) (ab205718; Abcam), and goat anti-mouse IgG H&L (HRP) (ab205719;

(17) The envelope protein of Zika virus interacts with apolipoprotein E early in the infectious cycle and this interaction is conserved on the secreted viral particlesVirology journalJuly 28, 2022Tréguier Y, Cochard J, Burlaud-Gaillard J, Lemoine R, Chouteau P, Roingeard P, Meunier JC, Maquart M.conjugated with HRP: donkey anti-mouse, donkey anti-rabbit and donkey anti-goat (ab6820, ab6802 and ab6885, respectively, Abcam) for 1 h at RT with shaking. The membranes were then subjected to a further three

(18) Selective Elimination of Senescent Fibroblasts by Targeting the Cell Surface Protein ACKR3International journal of molecular sciencesJune 10, 2022Takaya K, Asou T, Kishi K.incubated with the following secondary antibodies: donkey anti-goat IgG H&L (horseradish peroxidase; HRP) (ab6885; Abcam), goat anti-rabbit IgG H&L (HRP) (ab205718; Abcam), and goat anti-mouse IgG H&L (HRP) (ab205719;

(19) Ganoderma Lucidum Triterpenoids Improve Maternal Separation-Induced Anxiety- and Depression-like Behaviors in Mice by Mitigating Inflammation in the Periphery and BrainNutrientsMay 28, 2022Xue Mi, Gui-Rong Zeng, Jie-Qing Liu, Zhou-Song Luo, Ling Zhang, Xiao-Man Dai, Wen-Ting Fang, Jing Zhang, Xiao-Chun Chenmembranes were rinsed in TBST three times (10 min each) and reincubated in a secondary antibody (1:5000; ab6885, ab 6728, Abcam, Cambridge, UK) at room temperature for 60 min. Subsequently, the membranes were rinsed

(20) The transplantation of particulated juvenile allograft cartilage and synovium for the repair of meniscal defect in a lapine modelJournal of orthopaedic translationMarch 1, 2022Yan W, Maimaitimin M, Zhao F, Fan Y, Yang S, Li Y, Cao C, Shao Z, Liu Z, Hu X, et al.(Sigma, C8035) and SOX9 (Millipore Sigma, HPA001758) were used. Donkey anti-goat IgG H&L (HRP) (Abcam, ab6885), Goat anti-rabbit IgG H&L (HRP) (Abcam, ab6721) and anti-mouse IgG H&L (HRP) (Abcam, ab6789) were used

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1/24/2020One of the Best Anti-Goat Antibodies for Western Blot Matt SunThis is one of the best on the market despite the price being high.

Donkey Anti-Goat IgG H&L (HRP) from Abcam

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Donkey Anti-Goat IgG H&L (HRP) from Abcam

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